DIN EN ISO 15753:2006-12

This standard describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: - a general method, and - a method specific for coconut oil and short-chain vegetable oils. These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and pomace olive oil cannot be analysed using this method. The polycyclic aromatic hydrocarbons are extracted with an acetonitrile/acetone mixture followed by purification on C18 phase and then Florisil-phase bonded catridges. Determination of the content of the individual polycyclic aromatic hydrocarbons after separation is achieved by means of high-pressure liquid chromatography (HPLC) by measuring the fluorescence at various excitation and emission wavelengths.