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Normas DIN – AENOR
DIN EN ISO 17375:2005-05

DIN EN ISO 17375:2005-05

Animal feeding stuffs - Determination of aflatoxin B1 (ISO/DIS 17375:2005); German version prEN ISO 17375:2005

Aliments des animaux - Dosage de l'aflatoxine B1 (ISO/DIS 17375:2005); Version allemande prEN ISO 17375:2005

Futtermittel - Bestimmung von Aflatoxin B1 (ISO/DIS 17375:2005); Deutsche Fassung prEN ISO 17375:2005

Fecha Anulación:
2016-09 /Withdrawn
Equivalencias internacionales:

ISO/DIS 17375 (2005-03)

prEN ISO 17375 (2005-03)

Relación con otras normas DIN:

Reemplazada por: DIN EN ISO 17375:2006-09

This International Standard describes the determination of aflatoxin B1 in animal feed. This International Standard is based on a scientific publication of Werner. The limit of quantification of this International Standard has been demonstrated to be better than 0,5 µg/kg for aflatoxin B1 for a signal to noise ratio of 6. This method can be applied to the determination of aflatoxin B1 in animal feed with a fat content up to 50 %. The method involves extraction of a test with a solvent solution (acetone/water). The sample extract is filtered, diluted with water or phosphate buffered saline (PBS) to a specified solvent concentration, and a portion is applied on an immunoaffinity column containing antibodies specific to aflatoxin B1. The aflatoxin B1 is removed from the immunoaffinity column with neat methanol, and then quantified by reverse-phase liquid chromatography (RP-HPLC) with post column derivatisation (PCD) and fluorescence detection.
Aflatoxin, Animal feed, Animal husbandry, Chemical analysis and testing, Column chromatography, Derivatization, Detection limits, Determination of content, Extraction, Laboratory tests, Methods of analysis, Samples, Sampling methods
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