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Normas DIN – AENOR
DIN EN ISO 17375:2005-05

DIN EN ISO 17375:2005-05

Animal feeding stuffs - Determination of aflatoxin B1 (ISO/DIS 17375:2005); German version prEN ISO 17375:2005

Aliments des animaux - Dosage de l'aflatoxine B1 (ISO/DIS 17375:2005); Version allemande prEN ISO 17375:2005

Futtermittel - Bestimmung von Aflatoxin B1 (ISO/DIS 17375:2005); Deutsche Fassung prEN ISO 17375:2005

Fecha Anulación:
2016-09 /Withdrawn
Equivalencias internacionales:

ISO/DIS 17375 (2005-03)

prEN ISO 17375 (2005-03)

Relación con otras normas DIN:

Reemplazada por: DIN EN ISO 17375:2006-09

Resumen:
This International Standard describes the determination of aflatoxin B1 in animal feed. This International Standard is based on a scientific publication of Werner. The limit of quantification of this International Standard has been demonstrated to be better than 0,5 µg/kg for aflatoxin B1 for a signal to noise ratio of 6. This method can be applied to the determination of aflatoxin B1 in animal feed with a fat content up to 50 %. The method involves extraction of a test with a solvent solution (acetone/water). The sample extract is filtered, diluted with water or phosphate buffered saline (PBS) to a specified solvent concentration, and a portion is applied on an immunoaffinity column containing antibodies specific to aflatoxin B1. The aflatoxin B1 is removed from the immunoaffinity column with neat methanol, and then quantified by reverse-phase liquid chromatography (RP-HPLC) with post column derivatisation (PCD) and fluorescence detection.
Keywords:
Aflatoxin, Animal feed, Animal husbandry, Chemical analysis and testing, Column chromatography, Derivatization, Detection limits, Determination of content, Extraction, Laboratory tests, Methods of analysis, Samples, Sampling methods
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