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Normas DIN – AENOR
DIN EN ISO 23418:2020-10

DIN EN ISO 23418:2020-10

Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of foodborne bacteria - General requirements and guidance (ISO/DIS 23418:2020); German and English version prEN ISO 23418:2020 / Note: Date of issue 2020-09-11

Microbiologie de la chaîne alimentaire - Séquençage de génome complet pour le typage et la caractérisation génomique des bactéries dans les aliments - Exigences générales et recommandations (ISO/DIS 23418:2020); Version allemande et anglaise prEN ISO 23418:2020 / Attention: Date de parution 2020-09-11

Mikrobiologie der Lebensmittelkette - Vollständige Genomsequenzierung zur Typisierung und genomischen Charakterisierung von Bakterien in Lebensmitteln - Allgemeine Anforderungen und Leitfaden (ISO/DIS 23418:2020); Deutsche und Englische Fassung prEN ISO 23418:2020 / Achtung: Erscheinungsdatum 2020-09-11

Fecha:
2020-10 /Active
Equivalencias internacionales:

ISO/DIS 23418 (2020-09)

prEN ISO 23418 (2020-09)

Resumen:
This international standard specifies minimum requirements for generating and analyzing whole-genome sequence (WGS) data obtained from foodborne bacteria. These requirements are applicable to any sequencing platform or chemistry. This process may include the following stages: handling of bacterial cultures, genomic DNA Isolation, sequencing library preparation, sequencing, and assessment of raw DNA sequence read quality and storage, bioinformatic analysis, including methods such as high quality single nucleotide polymorphism (hqSNP) analysis, core genome and whole genome multi-locus genotyping (cgMLST, wgMLST), and bioinformatic pipeline validation; and Metadata capture and sequence repository deposition.
Keywords:
Analysis, Bacteria, Definitions, DNA, Food inspection, Food products, Genetics, Genomic information, Laboratories, Laboratory tests, Microbiology, Minimum requirements, Sequencing, Specification (approval), Typing procedures
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