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Normas DIN – AENOR
DIN EN 17550:2020-09

DIN EN 17550:2020-09

Animal feeding stuffs: Methods of sampling and analysis - Determination of carotenoids in animal compound feed and premixtures by high performance liquid chromatography - UV detection (HPLC-UV); German and English version prEN 17550:2020 / Note: Date of issue 2020-08-21

/ Attention: Date de parution 2020-08-21

Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von Carotinoiden in Mischfuttermitteln und Vormischungen für Tiere mittels Reverse Hochdruckphase-Flüssigchromatographie-UV Detektion (RP-HPLC-UV); Deutsche und Englische Fassung prEN 17550:2020 / Achtung: Erscheinungsdatum 2020-08-21

Fecha:
2020-09 /Active
Equivalencias internacionales:

prEN 17550 (2020-09)

Resumen:
This analytical procedure specifies a reverse phase high performance liquid chromatographic - UV detection (RP-HPLC-UV) method for the simultaneous determination of four authorised carotenoids in fish compound feed, namely astaxanthin (AXN), canthaxanthin (CXN), adonirubin (ADR) and astaxanthin dimethyldisuccinate (AXN DMDS), and of six authorised carotenoids in poultry feed, namely canthaxanthin (CXN), capsanthin (CSN), ethyl ester of beta-apo-8'-carotenoic acid (BACARE), citranaxanthin (CIXN), lutein (LUT) and zeaxanthin (ZEA) at levels ranging from 3 to 3.000 mg/kg (depending on the carotenoid). Beta-carotene (BCAR), which is authorised as colorant in canaries and as vitamin and provitamin in all animal species, was also added to the scope. The analytical procedure is fit for the purpose of quantitation of declared carotenoids and Iabelling confirmation. The procedure applies for natural and synthetic teed additives.
Keywords:
Animal feed, Animals, Carotenoids, Enzymatic methods, Fish food, Fishes, High performance liquid chromatography, HPLC, Investigation methods, Liquid chromatography, Mass concentration, Mixed provenders, Mixtures, Poultry, Sampling methods, Test performance, Ultraviolet, UV
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