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Normas DIN – AENOR
DIN CEN/TS 15633-2:2013-06;DIN SPEC 10700-1:2013-06

DIN CEN/TS 15633-2:2013-06;DIN SPEC 10700-1:2013-06

Foodstuffs - Detection of food allergens by immunological methods - Part 2: Quantitative determination of hazelnut with an enzyme immunoassay using monoclonal antibodies and bicinchoninic acid-protein detection; German version CEN/TS 15633-2:2013

Produits alimentaires - Détection des allergènes alimentaires par méthodes immunologiques - Partie 2: Détermination quantitative de la présence de noisette par un immuno-essai enzymatique à l'aide d'anticorps monoclonaux et détection des protéines avec l'acide bicinchoninique; Version allemande CEN/TS 15633-2:2013

Lebensmittel - Nachweis von Lebensmittelallergenen mit immunologischen Verfahren - Teil 2: Quantitative Bestimmung von Haselnuss mit einem Enzym-Immunoassayverfahren unter Verwendung von monoklonalen Antikörpern und Proteindetektion mit Bicinchoninsäure; Deutsche Fassung CEN/TS 15633-2:2013

Fecha:
2013-06 /Active
Equivalencias internacionales:

CEN/TS 15633-2 (2013-04)

Resumen:
This Technical Specification specifies an enzyme linked immunsorbent assay (ELISA) method for the determination of hazelnut from food samples. In the ELISA the antibodies bind to hazelnut proteins from the food sample. The result of the ELISA is given in mg hazelnut/kg (ppm) because the calibrators consist of an extract of whole hazelnut. Matrices like cereals, ice cream, cookies, chocolate, sausage, cottage cheese, yogurt and salad dressing were validated by spiking experiments with a carboxymethylcellulose-suspension containing hazelnut paste. The monoclonal antibodies, raised against the whole aqueous extract of hazelnut, detect proteins with approximate molecular weights of 14 kDa, 18 kDa, and 42 kDa. The antibodies detect the major thermostable allergen Cor a9 (11S storage protein). Both antibodies were evaluated by western blots with partially purified hazelnut extracts and purified allergenic proteins. The ELISA test kit method is commercially available. The performance has been validated by an in house validation performed by the manufacturer. All parameters of interest are indicated. In addition, the ELISA was successfully validated by a collaborative study in order to determine the interlaboratory reproducibility. This ring trial was organized by the working group established by the Federal Office of Consumer Protection and Food Safety (BVL) for the execution of § 64 of the German Food and Feed Code (LFGB) for the determination of hazelnut content in dark chocolate. Fourteen German laboratories participated in this collaborative study.
Keywords:
Allergenic, Allergies, Consumer protection, Determination procedures, ELISA, Enzyme assay, Food inspection, Food products, Food testing, Hazel-nuts, Methods of analysis, Nuts (food), Testing, Verification
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