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Normas DIN – AENOR
DIN EN 16204:2010-11

DIN EN 16204:2010-11

Determination of Lipophilic Algal Toxins (DSP-Toxins, Yessotoxins, Azaspiracids, Pectenotoxins) in Shellfish and Shellfish Products By LC-MS/MS; German version prEN 16204:2010 / Note: Date of issue 2010-11-08

/ Attention: Date de parution 2010-11-08

Bestimmung von lipophilen Algentoxinen (DSP-Toxine, Yessotoxine, Azaspiracide, Pectenotoxine) in Schalentieren und Schalentiererzeugnissen mit LC-MS/MS; Deutsche Fassung prEN 16204:2010 / Achtung: Erscheinungsdatum 2010-11-08

Fecha Anulación:
2012-08 /Withdrawn
Equivalencias internacionales:

prEN 16204 (2010-11)

Relación con otras normas DIN:

Reemplazada por: DIN EN 16204:2012-08

Resumen:
A multireference method is described for the qualitative detection and the quantitative determination of lipophilic toxins (fat-soluble algal toxins produced by dinoflagellates) in mussels and mussel products by LC-MS/MS.The detection limit for DSP toxins, azaspiracids and pectenotoxins was determined to be 6 µg/kg mussel meat and for yessotoxins 10 µg/kg mussel meat.Quantitative determination of okadaic acid, pectenotoxin2 (PTX2), azaspiracid1 (AZA1), and yessotoxin (YTX) can be carried out directly by means of standard substances available commercially. Assuming an equal response factor, okadaic acid is used for the indirect quantitative determination of the two dinophysis toxins dinophysis toxin-1 (DTX1) and dinophysis toxin-2 (DTX2); likewise azaspiracid1 (AZA1) is used for the indirect quantitative determination of azaspiracid2 (AZA2) and azaspiracid3 (AZA3), while YTX is used for homoyessotoxin, OH-yessotoxin and OH-homo-yessotoxin, and PTX2 for PTX1.The limit of quantification for DSP toxins, azaspiracids and pectenotoxins was determined to be 20 µg/kg mussel meat and for yessotoxins 35 µg/kg mussel meat.By means of hydrolysis, the esters of okadaic acid, DTX1 and DTX2 can also be determined quantitatively. Subsequent to preliminary tests, this method is also applicable to further lipophilic marine biotoxins such as gymnodimine or spirolides, if standards are available. Likewise, on condition that preliminary tests are carried out, this method can also be used for the analysis of crustaceans and other bivalves as well as for products from those two.
Keywords:
Algae, Algal toxins, Chemical analysis and testing, Determination of content, Detrimental to health, Extraction, Food inspection, Food products, Food testing, High performance liquid chromatography, HPLC, Inter-laboratory tests, Investigations, Lipophile, Mycotoxin, Reference methods, Shellfish, Testing, Toxin, Verification
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