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Normas BSI – AENOR
19/30404344 DC

19/30404344 DC

BS EN ISO 14501. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

2019-12-18 /Definitive
Equivalencias internacionales:

ISO/DIS 14501


This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 µg/kg for milk powder and LOD is 0,005 µg/kg for liquid milk. The limit of quantification(LOQ) is 0,1 µg/kg for milk powder and LOQ is 0,01 µg/kg for liquid milk.

The method is also applicable to low fat milk, skimmed milk, low fat milk powder and skimmed milk powder.


The method described in this protocol requires the use of solutions of aflatoxin M1. Aflatoxins are carcinogenic to humans. Attention is drawn to the statement made by the International Agency for Research on Cancer (WHO)[1,2].


Protect the laboratory in which the analyses are performed adequately from daylight and keep aflatoxin standard solutions protected from light, e.g. by using aluminium foil.


The use of non-acid-washed glassware (e.g. tubes, vials, flasks, beakers, syringes) for aqueous aflatoxin solutions may cause loss of aflatoxin.
Moreover, brand new laboratory glassware, before coming into contact with aqueous solutions of aflatoxin, should be soaked in dilute acid (e.g. sulfuric acid, 2 mol/l) for several hours, then rinsed well with distilled water to remove all traces of acid (check to ensure pH is in the range 6 to 8).


Use decontamination procedures for laboratory wastes such as solid compounds, solutions in organic solvents, aequeous solutions and spills, and for glassware coming into contact with carcinogenic materials. Suitable decontamination procedures have been developed and validated by the International Agency for Research on Cancer (WHO)[1,2].

Immunoassay, Specimen preparation, Milk, Dried milk, Chemical analysis and testing, Food products, Determination of content, Test equipment, Test specimens, Aflatoxin, Affinity chromatography, Food testing, Liquid chromatography, Dairy products
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