BS ISO 23443. Infant formula and adult nutritionals. Determination of lutein, ß-carotene and lycopene by reversed phase ultra high performance liquid chromatography (RP-UHPLC)
67.050 - General methods of tests and analysis for food products
This document specifies a method for the quantitative determination of ß-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed phase ultra high performance liquid chromatography and UV-visible detection. The application range runs from 1 µg/100 g to 1 500 µg/100 g for lycopene and from 1 µg/100 g to 2 250 µg/100 g for ß-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 µg/100 g and the limit of quantification (LOQ) was 0,3 µg/100 g for each carotenoid. The method is not applicable to materials that contain measurable levels of ß-apo-8'-carotenal. The reproducibility data meets the requirements given in Reference  and .
The informative Annex C of this document specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference .
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