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ASTM D4576-16

ASTM D4576-16

Standard Test Method for Mold Growth Resistance of Wet Blue and Wet White

2016-12-27 /Active
Significance and Use:

4.1 This test method provides a technique for evaluating mold growth resistance characteristics of Wet Blue and Wet White, and should assist in the prediction of storage time before molding occurs.

4.2 The degree of correlation between this test and commercial quantities of Wet Blue and Wet White in storage or shipment situations, or both, has not been fully determined.


1.1 This test method covers the determination of mold growth resistance of Wet Blue and Wet White subject to storage and shipping requirements and intended for use in leather manufacturing. This test method may not be suitable to evaluate fungicides that are inactivated by proteins. This includes alkyldimethylbenzyl ammonium chlorides.

1.2 Conclusions about mold growth resistance are drawn from the results by comparing the test with a simultaneously run control of known resistance. Success or failure is determined by the amount of mold growth relative to the control.

1.3 To allow use of this test method by any laboratory, flexibility has been permitted in times, temperature, and humidity of incubation, inoculum, hide sampling area, and choice of control. These may be adjusted to fit local conditions but must be standardized.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

agar plate; blue stock; inoculum; mold; Wet Blue; Wet White;
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