Standard Guide for Calibrating Reticles and Light Microscope Magnifications
4.1 These methods can be used to determine magnifications as viewed through the eyepieces of light microscopes.
4.2 These methods can be used to calibrate microscope magnifications for photography, video systems, and projection stations.
4.3 Reticles may be calibrated as independent articles and as components of a microscope system.
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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