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ASTM E2471-05(2011)e1

ASTM E2471-05(2011)e1

Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets

2016-12-01 /Historical
Superseeded by:
Significance and Use:

This test method provides for rapid screening of antimicrobial treatments located in or on the carpet face fiber or incorporated into the backing structure of the carpet (or both).

This test method simulates actual use conditions that may occur on carpets (for example, food and beverage spills, soiling from foot traffic, prolonged moisture exposure).

This test method provides a means to screen for activity and durability of an antimicrobial treatment under conditions of organic loading.

This test method provides for the simultaneous assessment of multiple carpet components for antimicrobial activity.

Carpets may be cleaned prior to testing with this test method in order to assess the durability of the antimicrobial effect.


1.1 This test method is designed to evaluate (qualitatively) the presence of antimicrobial activity in or on carpets. Use this test method to qualitatively evaluate both antibacterial and antifungal activity.

1.2 Use half strength (nutrient and agar) tryptic soy agar as the inoculum vehicle for bacteria and half strength potato dextrose agar as the inoculum vehicle for mold conidia. Use of half strength agars may reduce undue neutralization of an antimicrobial due to excessive organic load.

1.3 This test method simultaneously evaluates (both visual and stereo-microscopic) antimicrobial activity both at the fiber layer and at the primary backing layer of carpet.

1.4 Use this test method to assess the durability of the antimicrobial treatments on new carpets, and on those repeatedly shampooed or exposed to in-use conditions.

1.5 Knowledge of microbiological techniques is required for the practice of this test method.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

antimicrobial; bacteria; carpet; fungi; low solubility preservative; mold; Carpets; Antimicrobial formulations/testing; 97.150 (Non-textile floor coverings)
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