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ASTM E2524-08

ASTM E2524-08

Standard Test Method for Analysis of Hemolytic Properties of Nanoparticles

2013-10-02 /Historical
Superseeded by:
Significance and Use:
p>This test method is one of a series of tests listed in Practice F 748 and ISO 10993-4 to assess the biocompatibility of materials contacting blood in medical applications.

This test method is similar to Practice F 756 but modified to accommodate nanoparticulate materials.


1.1 This test method covers assessing the effect of nanoparticulate materials on the integrity of red blood cells.

1.2 This test method uses diluted whole blood incubated with nanoparticulate material and the hemoglobin released from damaged red blood cells is determined.

1.3 This test method is similar to Practice F 756 with the volumes reduced to accommodate nanoparticulate material.

1.4 This test method is part of the in-vitro preclinical characterization and is important for nanoparticulate material that will contact the blood in medical applications.

1.5 The values given in SI units are to be considered as the standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

hemolysis; nanoparticulate; plasma; whole blood
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