This test method is an important part of the final phase of study in the development of mosquito repellents for personal use.
This test method is primarily designed to simulate a situation in which a person treated with a repellent is exposed to natural populations of attacking mosquitoes.
The simplicity of the test offers flexibility under a relatively wide range of circumstances and geographical locations. By following this test method, international testing with a variety of vector mosquito populations is no more difficult to accomplish than tests with various domestic species.
A number of people test topical applications of a repellent for the following reasons:
4.4.1 To determine how long the repellent is effective;
4.4.2 To establish the effective dosage range;
4.4.3 To establish the range of effectiveness on several mosquito genera and species in a number of geographical areas; and
4.4.4 To identify the material in terms of odor, staining capability, plasticizing effect, and oiliness or greasiness.
No repellent should be tested on humans without the written consent of the test volunteers (hereafter referred to as test subjects) and prior approval of competent authority, as designated in applicable laws and regulations governing experimentation on humans.