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ASTM E1482-04

ASTM E1482-04

Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations

2012-11-12 /Historical
Superseeded by:
Significance and Use:

This test method is to be used for the removal of virucidal agents from agent-virus mixtures, or from agent-neutralizer-virus mixtures, after the contact period and before the inoculation of these mixtures into host systems for assay of infectivity.

The purpose of the test method is to reduce the concentration of agents and neutralizers in order to permit the evaluation of viral infectivity at dilutions that would otherwise be toxic to the host.

The test method is applicable to the testing of liquid and pressurized disinfectant products.

This test method is compatible with organic soil loads, hard water, disinfectants containing organic solvents, and chemical neutralizers.


1.1 This test method covers the use, in conjunction with evaluations of the virucidal efficacy, of disinfectant solutions or pressurized disinfectant spray products intended for use on inanimate nonporous environmental surfaces or for other special applications. The test method may be employed with all viruses and host systems.

1.2 This test method should be performed only by persons trained in microbiology and virology.

1.3 This test method utilizes gel filtration technology. The effectiveness of the test method is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns and centrifugation conditions. The effectiveness of this test method is maximized by investigator practice and experience with gel filtration techniques.

1.4 This test method will reduce, but not necessarily eliminate, disinfectant toxicity while preserving the titer of input virus.

1.5 The values stated in SI units are to be regarded as the standard.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

cytotoxicity; disinfectant; gel filtration; neutralization; tissue culture; virucidal; virucidal neutralization method
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